Background: Benign thyroid nodules (BTN) are frequently diagnosed as papillary thyroid carcinoma (PTC), leading\nto unnecessary treatment. We found that plasma lncRNA DLG1-AS1 was upregulated in PTC patients but not in BTN\npatients and healthy controls.\nMethods: In this study DLG1-AS1 and miR-199a-3p in plasma of both PTC patients and BTN patients were detected\nby qPCR. ROC curve analysis was performed for diagnostic analysis. Overexpression experiments were performed to\nanalyze the interaction between DLG1-AS1 and miR-199a-3p. CCK-8 assay was performed to analyze cell\nproliferation.\nResults: In this study, upregulation of DLG1-AS1 distinguished PTC patients from BTN patients and healthy controls.\nPlasma miR-199a-3p was downregulated in PTC patients compared with healthy controls and BTN patients. Plasma\nlevels of miR-199a-3p were inversely correlated in PTC patients, but not in BTN patients and healthy controls. miR-\n199a-3p overexpression failed to significantly affect DLG1-AS1, while DLG1-AS1 overexpression resulted in\ndownregulated miR-199a-3p, In addition, DLG1-AS1 overexpression promoted the proliferation of PTC cells. miR-\n199a-3p overexpression played an opposite role and attenuated the effects of DLG1-AS1 overexpression.
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